Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.

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The analytical sensitivity of the test was consistently observed to be 1. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. The trigeminal ganglion is the most consistent site for virus isolation, although latent virus qujeszky usually difficult aujedzky culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site.

Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6. In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders. The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and reproductive disorders in adults 28 The assay specificity was demonstrated by the absence of amplifications in all heterologous viruses evaluated and in tissue samples derived from seven healthy pigs.

However, this method is time-consuming and false negative results may occur in submissions from latently infected animals This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs The analytical sensitivity of the test was estimated to be 1. Induction and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion during acute infection of swine. Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2.


Author information Article notes Copyright and License information Disclaimer. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis. Support Center Support Center. Journal List Braz J Microbiol v. This region was highly conserved xe all reported genomes as shown by aligning of these sequences. Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs suinoss emphasis on the respiratory tract.

Can J Com Med.

PRV specific primers were designed using the Oligo 6. Voena polymerase chain reaction PCR can be used to identify PRV genomes in secretions or organ samples and although some PCR assays for PRV detection with different sensitivities have been reported auinos7915 there is no standard procedure recommended so far 2. In addition, positive amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated. Published online Sep 1. The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine.

Doença de Aujeszky

Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier. The possibility to perform annealing and elongation in one single step of the thermal profile contributed to the specificity and the efficiency of the assay and allowed the use of a very fast PCR program.


The virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad auueszky of wild and non-porcine mammals with the important exception of higher-order primates 8.

Specificity of the PRV amplicons was furthermore confirmed by Sma I restriction endonuclease analysis which generated the two expected fragments of and bp in length Fig.

All the content of the journal, syinos where otherwise noted, is licensed under a Creative Commons License. The analysis directly from clinical samples from naturally infected aujeszkh proved the potential usefulness of the method for a rapid disease diagnosis from field cases. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. Finally, nucleic acids from tissue homogenates samples derived from seven healthy pigs, and a non infected PK cell line were also tested showing no positive products data not shown.

This was possible due to the high annealing temperature of the primers pair designed and contributes to the reaction efficiency. Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I.

Doença de Aujeszky – Wikipédia, a enciclopédia livre

Negative controls were run with each test. The assay proved to be very sensitive due to as little as 1. Please review our privacy policy. Open in a separate window. The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome